How does the qPCR test detect Candida?

The qPCR test detects the presence or absence of specific DNA sequences unique to Candida by amplifying these sequences when they are present in a sample. This highly sensitive and specific technique provides reliable indications of the presence of Candida and involves several key steps:

Extraction and Isolation: Initially, DNA is carefully extracted and isolated from the sample, preparing it for testing.

Amplification: In the qPCR machine, specially designed DNA primers that match unique sequences of Candida are used. These primers locate and bind to their corresponding DNA sections within the sample. The qPCR machine then amplifies these sections of DNA across multiple cycles, exponentially replicating the target DNA sequence.

Detection and Quantification: Throughout the amplification process, each cycle is monitored in real time using a fluorescence marker. This marker binds to the amplified Candida DNA sequences, emitting a signal that intensifies as more DNA is produced. This enables the determination of whether the pathogen is present or not.