When and why should I use a qPCR addition to the shotgun sequencing test?

The qPCR test is provided alongside shotgun sequencing for several reasons:

• Sensitivity and Specificity: qPCR is exceptionally sensitive and specific, making it highly reliable for detecting the presence of specific organisms like Candida and H. pylori. This is important when precise identification and confirmation of these pathogens are required, especially in cases where their presence is critical to determining the correct treatment plan.
• Rapid Results: qPCR tests typically have a faster turnaround time compared to shotgun sequencing. This rapid delivery of results may be useful in clinical settings.
• Complement Existing Data: For patients who have undergone comprehensive microbial community analysis through shotgun sequencing, adding a qPCR test can help confirm and validate the findings, particularly in scenarios where specific pathogens need to be monitored or when the shotgun results are inconclusive. This complementary approach ensures greater accuracy and confidence in clinical decision-making.