How does the qPCR test detect Candida and H. pylori?

The qualitative qPCR test detects the presence or absence of specific DNA sequences unique to Candida and H. pylori by amplifying these sequences if they exist in a sample. This highly sensitive and specific technique offers reliable indications of the presence of these pathogens and involves several key steps:

Extraction and Isolation: Initially, DNA is carefully extracted and isolated from the sample, preparing it for testing.

Amplification: In the qPCR machine, specially designed DNA primers that match the unique sequences of Candida and H. pylori are used. These primers locate and bind to their corresponding DNA sections within the sample. The qPCR machine then amplifies this section of DNA across multiple cycles, exponentially replicating the target DNA sequence.

Detection and Quantification: Throughout the amplification process, each cycle is monitored in real-time by a fluorescence marker. This marker binds to the amplified DNA sequences of Candida and H. pylori, emitting a signal that intensifies as more DNA is produced. This enables the determination of whether the pathogen is present or not.