Tiny Health uses shotgun metagenomic sequencing, which offers:
Advantages over qPCR:
- Detects entire microbial community vs. 20-50 targets
- Species- and strain-level resolution
- Quantifies relative abundance across the entire microbiome
- Reveals functional gene capacity
Advantages over 16S rRNA sequencing:
- Higher resolution of the microbial community
- Species-level identification (16S typically stops at genus)
- Functional pathway analysis (16S infers function, shotgun measures genes)
- More accurate quantification
- Better resolution of the microbiome
For vaginal testing: Shotgun sequencing is rare in clinical vaginal microbiome assessments. Most vaginal tests use culture or limited qPCR panels. Tiny Health provides comprehensive species identification, giving a full picture of the microbial environment, and reports on the vaginal community state type of the sample.
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